Virulence of Fusarium species to alfalfa seedlings

Virulence of Fusarium species to alfalfa seedlings

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In in vitro conditions, virulence of 91 isolates of species Fusarium genus (F.oxysporum, F.solani, F.acuminatum, F.

equiseti, F.arthrosporioides, F.prolifera- tum, F.avenaceum, F.

semitectum, F.tricinctum, F.sporotrichioides and F.graminearum) towards alfalfa seedlings was investigated.

Isolates of investigated species originated from diseased alfalfa plants collected at four locations in Serbia based on symptoms of wilting caused by Fusarium and root rotting.Pathogenicity and virulence of Toys investigated isolates of Fusarium spp.were determined by visual evaluation of inoculated seedlings of cultivar K28 in Car Model Kit laboratory conditions.All isolated of investigated species had pathogenic effect on alfalfa seedlings which expressed symptoms such as necrosis of root, moist rotting and "melting of seedlings".

Colour of necrotic root tissue varied from light brown, brown lipstick red to explicit black, depending on the Fusarium species.Strong virulence was established in 48 isolates, medium virulence in 31 and weak virulence in 12 isolates.